Unexpected mutations after CRISPR?Cas9 editing in vivo
Kellie A Schaefer, Wen-Hsuan Wu, Diana F Colgan, Stephen H Tsang, Alexander G Bassuk & Vinit B Mahajan
AffiliationsCorresponding authors
Nature Methods 14, 547?548 (2017) doi:10.1038/nmeth.4293
Published online 30 May 2017
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To the Editor:
CRISPR?Cas9 editing shows promise for correcting disease-causing mutations. For example, in a recent study we used CRISPR-Cas9 for sight restoration in blind rd1 mice by correcting a mutation in the Pde6b gene1. However, concerns persist regarding secondary mutations in regions not targeted by the single guide RNA (sgRNA)2.…
